Research Output | Dar Al-hikmah Library | IIUM Only
Plant-esterase from wheat flour was purified via aqueous two-phase system (ATPS). The enzyme crude extract was first filtered through a polyethersulfone OmegaTM ultrafiltration membrane, followed by two-step ATPS with PEG1000, NaH2PO4 and (NH4)2SO4. Based on previous studies, the first ATPS consisting of 27.0% PEG1000 and 13.0% NaH2PO4 (w/w) was pe