Abstract: Use of computational methods to model and design new enzymes with enhanced functionalities is an emerging technology. In this work endoglucanase obtained from Fusarium oxysporum is modeled using homology modeling, and the dynamics of this enzyme in vacuum and water medium is examined to analyze the effect of the environment on the structu

This research has used Molecular Dynamics (MD) techniquess as an in silico method of correlating the experimental studies done on GUS enzyme with computational study and has analyzed and identified the structural factors responsible for thermostability of this enzyme. GUS from E. coli is heat labile and inhibited by detergents and products, which h

Homology modeling is a powerful tool in predicting the three dimensional (3D) structure of a protein using a solved structure within the same family as template. Compared to other methods such as X-ray crystallography and NMR, homology modelling has the advantage of being a fast yet reliable technique in solving proteins’ 3D structures, starting

Acid β-glucosidase (GlcCerase) is a lysosomal enzyme, which is important in biodegradation of blood cells in human body. Mutation of GlcCerase will lead to Gaucher disease; the most common lysosomal storage disease. The current available treatments for Gaucher disease are enzyme replacement therapy and substrate reduction therapy; and both are cos

Background: Smoking among Malaysian female 13 to 18 years is 4.8% (NHMS 2, 1996). However, assessment of knowledge and perception regarding smoking in this group is lacking. Objectives: To determine smoking-related knowledge, attitude and behaviour among female adolescent smokers. Methods: 56 female adolescent smokers selected randomly at a shoppin

In the mid-1990s, the East Asian countries experienced severe financial crisis that were followed by deep economic downturns. A variety of methodologies have been used to understand the nature of the Asian financial crisis. However, the impact of the 1997 Asian financial crisis on the efficiency of the financial industry has yet to be critically ex

Based on the theory of constructivism, this study identified 14 year old’ schematized meaning of the concept of percent and how the children use them in solving the related problems. Data incorporating both verbal and non-verbal behaviour was gathered from seven 14 year-olds based on five successive clinical interview sessions involving several p

Petroleum sourced fuels are recognized as unsustainable energies owing to depleting supplies and the accumulation of carbon dioxide in the atmosphere cause by the contribution of these fuels. Renewable sourced fuels, biofuels are necessary for environmental and economic sustainability. In biofuels production such as bioethanol, Fusarium oxysporum i

Computational chemistry is a branch of chemistry that uses principles of computer science to assist in solving chemical problems. Structures and properties of molecules can be calculated when theoretical chemistry is incorporated into efficient computer programs. Its necessity arises from the well-known fact that apart from relatively recent result

Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of ultrasound irradiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of ultrasound energy on decellularization of cartilage tissue. T